Mapping ends of crRNAs in total RNA and crRNAs associated with Csa, Cst, Cmr complexes. 5′ and 3′ end analysis of all sequenced crRNAs in untreated S100 extract (total RNA) and crRNAs associated with Csa (Csa2, Cas3″), Cst (Cst2, Cas5t), and Cmr (Cmr2) complexes. The percentage of crRNAs starting and ending at a specific point is marked on the y-axis. The nucleotides are counted relative to their position from the guide-repeat junction. The nucleotides upstream of the guide-repeat junction are counted from −1 and those downstream are counted from +1. The peaks in the 5′ repeat region are shown in blue. The peaks downstream from the 5′ repeat-guide junction are shown in orange. The schematic on top indicates where the peaks map (repeats black and guides green).
