Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus

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FIGURE 4.
FIGURE 4.

Northern blot analysis of crRNAs associated with Csa, Cst, and Cmr complexes. Northern blotting of RNAs extracted from Pfu S100 extract (T), preimmune pellets (PI), and immune pellets (I) of Csa (Csa2, Cas3″), Cst (Cst2, Cas5t), and Cmr (Cmr2). The blots were probed for the first crRNA of locus 7 (7.01). Asterisk indicates the prominent 45-nt crRNA band detected in all three complexes. Radiolabeled decade marker in nucleotides (M).

This Article

  1. Published in Advance April 22, 2015, doi: 10.1261/rna.049130.114 RNA 21: 1147-1158

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