Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus

Western blot analysis of Cas proteins associated with Csa, Cst, and Cmr complexes. All five immunopurified samples were blotted and probed with each of the five immune antibodies (Csa2, Cas3″, Cst2, Cas5t, Cmr2) individually. The first lane of each blot is untreated Pfu S100 extract used as a reference. An asterisk indicates the expected position of antigens based on their molecular weights. Molecular weights are indicated in kilodaltons (kDa).