Genetic and structural analysis of the essential fission yeast RNA polymerase II CTD phosphatase Fcp1

Synthetic genetic interaction of Fcp1 mutations with null mutants of Ssu72. Haploid fcp1–hygMX strains were mixed with ssu72⁺–kanMX (WT), ssu72::kanMX (Δ), or ssu72-(C13S)–kanMX strains (Schwer et al. 2012) of the opposite mating type on malt agar for mating and sporulation, after which hyg^R kan^R haploids with the indicated genotypes were recovered. Exponentially growing cultures of the strains specified were adjusted to A₆₀₀ of 0.1 and aliquots of serial fivefold dilutions were spotted to YES agar and incubated at the indicated temperatures.