Mutational analysis of the essential N domain. (A) Alignment of the primary structures of the Fcp1 N domains of Schizosaccharomyces pombe (Spo), Candida albicans (Cal), and Aspergillus fumigatus (Afu). Positions of amino acid side chain identity/similarity in all three proteins are indicated by •. Conserved residues targeted for alanine scanning in SpFcp1 are denoted by ▾. A putative CHCC metal-binding motif is demarcated by a bracket. The lethal Δ20 truncation is denoted by . (B) The indicated fcp1 alleles were tested for complementation in vivo. Lethal alleles are denoted by −. +++ Indicates growth on YES agar indistinguishable from wild-type fcp1+. Y105A cells grew slowly (scored as +) and formed tiny colonies on YES agar.
