Discovery and investigation of the GAIT translational control system
- Department of Cellular and Molecular Medicine, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio 44195, USA
- Corresponding author: foxp{at}ccf.org
This extract was created in the absence of an abstract.
My laboratory has been studying the GAIT translational control pathway for almost exactly 20 years. We stumbled into the translation field entirely by accident, without forethought, and with little knowledge or experience. Barsan Mazumder and Chinmay Mukhopadhyay, two fellows in my lab investigating ceruloplasmin (Cp) expression, observed that interferon (IFN)-γ induced monocytic cell expression of Cp, a secreted acute phase protein, as well as its transcript. The inducible level of Cp mRNA and Cp protein in the conditioned medium both flattened after about 12 h. At first glance this was not a surprising finding. But we soon realized the result was, in fact, unusual and suggested a mechanism beyond simple transcription and translation. Because Cp is a highly stable, secreted protein, we expected it to accumulate continuously in the conditioned medium in the presence of a constant amount of Cp mRNA. We were astute enough to recognize the interesting disparity, …
