Prp40 pre-mRNA processing factor 40 homolog B (PRPF40B) associates with SF1 and U2AF⁶⁵ and modulates alternative pre-mRNA splicing in vivo

Colocalization of PRPF40B with the splicing factors SF1 (A) and U2AF⁶⁵ (B). Immunofluorescence analysis of HeLa (lower panels) and HEK293T (upper panels) cells dual-labeled with antibodies to detect PRPF40B and SF1 (A) or U2AF⁶⁵ (B). The merged images represent a superimposition of PRPF40B (green) and SF1 or U2AF⁶⁵ (red) labeling. Line scans showing the local intensity distribution of dual staining are shown to the right of the panels. Bars in the merged panels indicate the position of the line scans. Bars, 3 mm.