PRPF40B is enriched in splicing factor-rich nuclear speckles. (A) Endogenous PRPF40B was detected with IgG-purified polyclonal antibodies. HEK293T and HeLa cells were dual-stained with antibodies to detect PRPF40B (green) and SRSF2 (red), and merged images are shown. Line scans with local intensity distributions are represented at the right. The bar in the merged panel indicates the position of the line scans. Bar, 3 mm. (B) Immunofluorescence analysis was performed to determine the location of PRPF40B. HEK293T or HeLa cells were transfected with an EGFP-tagged PRPF40B construct (green) and labeled with antibodies directed against the essential splicing factor SRSF2 (red) to stain nuclear speckles. Individual staining and the superimposition of both images (merge) are shown. Line scans showing local intensity distributions of dual staining are represented to the right of the panels. A bar in the merged panel indicates the position of the line scans. Bar, 3 mm.
