(A) Alignment of CTE homologs in intron 10 of the human (Hs) NXF1, the zebrafish (Dr) NXF1, and the Latimeria (Lc) NXF1 genes. The top line shows the sequence of human NXF1 CTE. A dot (.) shows that the nucleotide is same as the one in the top line. A dash (-) shows that there is a gap in the alignment. Regions indicated below the sequences correspond to CTE secondary structures involved in stem formation as shown in C. (B) Schematic representation of the NXF1 genes from human (ENSG00000162231 11:62559595-62573774:-1), zebrafish (ENSDARG00000055076 21:26058501-26071321:-1), and Latimeria (ENSLACG00000001232 JH126593.1:10100-40448:-1). Open boxes represent the 5′ and 3′ untranslated regions. Vertical bars represent exons. Each intron is numbered and the respective length of each gene in kilobases is shown. Intron 10, containing the CTE, is 1801 nt long in the human gene, 3995 nt long in zebrafish, and 3426 nt long in Latimeria. (C) Secondary structures of CTE core sequences from human, zebrafish, Latimeria, and MPMV. Nucleotide variations from the human CTE are shown in bold and underlined.
