MiR-130a could play a regulatory role in energy metabolism in hepatocytes. (A) The mRNA expression of G6Pase (1.0 kb) and PEPCK (1.8 kb) was reduced in stable miR-130a expressing HepG2 cells by Northern blot analysis. (B) Reduced protein expression of PEPCK (69 kDa) and G6Pase (40 kDa) was also detected by Western blot analysis. We noted an increased level of glucokinase (GCK) (52 kDa) in stable miR-130a expressing HepG2 cells, and an increased level of pyruvate kinase (PKLR) (58 kDa) in stable miR-130a expressing HuH-7 cells. (C) The levels of PKLR and GCK specific mRNAs were increased in stable miR-130a expressing cells by RT-qPCR analysis. (D) The protein expression of PGC1α and gluconeogenic enzymes PEPCK and G6Pase in HBV-producing HepG2 cells (UP7-4 and UP7-7) was increased by Western blot analysis.
