Regulatory activity of endogenous miR-10404/miR-ITS1-5p. Luciferase sensors bearing no target site or a two tandem copy of target sequences that is perfectly complementary to miR-10404/miR-ITS1-5p were cotransfected with 2′-O-methylated RNA oligonucleotide inhibitor against mature miR-288, miR-10404/miR-ITS1-5p, or hp-CG4068B. The sensors were derepressed only when the cognate inhibitor was cotransfected. Note that stronger derepression was observed in Kc167 cells consistent with the expression levels of miR-10404/miR-ITS1 in these cell lines. The columns and error bars depict means and standard deviations, respectively (N = 4). Asterisks indicate statistically significant differences compared with the values with the control miR-288 antisense oligonucleotide (P < 0.05, t-test).
