A potential role for hnRNP AB in hormone-induced exon skipping. (A) Hormone-induced gene expression changes of genes encoding proteins involved in splicing and its regulation. The direction of the change (up- or down-regulation) upon hormone treatment is indicated. (B) Frequency of hnRNP AB binding motifs relative to exons that are included or skipped upon hormone treatment, alternative exons (AE), or control exons. Frequencies correspond to segments of 150 bp upstream, downstream, or at the exons. P-values for motif enrichment in each region are also represented. (C) Schematic representation of Genome browser view of the hnRNP AB gene displaying tracks of PR binding before and 60 min after R5020 treatment. (D) RT-qPCR analysis of hnRNP AB mRNA levels 6 h after R5020 treatment or after vehicle treatment, relative to GAPDH mRNA levels in control cells or cells depleted of hnRNP AB by RNAi. (E) Percentage of inclusion of the indicated exons measured by semiquantitative RT-PCR under the same conditions as in D. (*) P-value 0.05 > P > 0.01.
