Switches in the distribution of nucleosome profile clusters upon hormone stimulation. (A) Fraction of exons (total, R5020-induced, and R5020-skipped with a minimum ΔPSI of 0.15) that switch or not between nucleosome profile clusters upon hormone stimulation. (B) Fraction of exons in each class that switch (light colors) or not (dark colors) between nucleosome profile clusters upon hormone stimulation. Color codes are as in Figure 4. Values are represented for all, R5020-induced, and R5020-skipped exons. (C–E) Distribution of exons in nucleosome profile clusters before (solid bars) and after (striped bars) progesterone treatment. Values are represented for all (C), R5020-induced (D), and R5020-skipped (E) exons. Color codes representing each profile cluster are as in Figure 4. Figures between brackets indicate exon numbers in each category. Distribution of nucleosome profile cluster switches for R5020-included (F) and R5020-skipped (G) exons. Classes are indicated by schemes of the profiles. The fraction and number of exons displaying each switch (out of the total number of exons switching profile) is indicated. Single and double black dots indicate switches between categories which are more frequent in hormone-regulated exons compared with all alternative exons. (H,I) Genome browser visualization of MNase profiles before (blue) and after (red) hormone induction around two R5020-regulated exons. Maximum MNase peak values are also represented. Both exons change in nucleosome cluster type upon hormone induction. R5020-included exon in the gene PAPOLA, switches from cluster A in uninduced (T0) cells to cluster B 60′ after R5020 induction. R5020-skipped exon in the gene AGBL5, switches from cluster B in uninduced (T0) cells to cluster C 60′ after R5020 induction.
