Arginine methylation of SFPQ/PSF does not affect binding to NONO. (A,B) Methylation assays were performed in conjunction with the addition of tritiated S-adenosyl-L-methionine ([3H] AdoMet) to detect methylation of (A) recombinant SFPQ using PRMT 1, 3, 4, and 6 and (B) endogenous SFPQ using PRMT1. (C) Antibody enrichment of the SFPQ/NONO complex using total extracts from +/− AdOx treated cells. Eluted proteins were analyzed by SDS-PAGE stained with Coomassie blue. (D) Co-IP of FLAG-SFPQ in the presence of RNAse A from 293T cells treated with AdOx/AMI-1 as indicated. Total extract (1% of input) and eluted proteins were analyzed by Western blotting with anti-FLAG and anti-NONO. (E) Co-IP of FLAG-SFPQ in the presence of RNAse A from 293T cells treated with AdOx as indicated. Total extract (1% of input) and eluted proteins were analyzed by Western blotting with anti-FLAG, anti-NONO, and anti-Chtop.
