A simple real-time assay for in vitro translation

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FIGURE 5.
FIGURE 5.

(A) SNAPf synthesis curve for wild-type and mutant ribosomes. SNAPf concentrations are data points with error bars, and the first-order kinetic fits are solid lines. Reaction conditions same as Figure 3A. (B) Differential analysis for SNAPf synthesis data. (C) Comparison of wild-type and mutant activities calculated from the first-order kinetics model and differential analysis.

This Article

  1. Published in Advance December 18, 2014, doi: 10.1261/rna.047159.114 RNA 21: 296-305

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