A simple real-time assay for in vitro translation

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FIGURE 3.
FIGURE 3.

(A) Fluorescence curves of in vitro SNAPf translation by wild-type and mutant ribosomes. Points with error bars are fluorescence data, and solid lines are fits to the first-order kinetics model. All fits were validated by bootstrap analyses. Reaction conditions: 500 nM ribosomes, 2 μM mRNA, and 10 μM CBG-488-TQ2. (B) Differentials of fluorescence curves.

This Article

  1. Published in Advance December 18, 2014, doi: 10.1261/rna.047159.114 RNA 21: 296-305

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