A simple real-time assay for in vitro translation

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FIGURE 1.
FIGURE 1.

(A) Comparison of fluorescence generated in the presence or absence of SNAPf mRNA. Reaction conditions: 100 nM ribosomes, 0 or 100 nM mRNA, and 500 nM CBG-549-QSY7. (B) Fluorescence response over a range of wild-type ribosome concentrations. Reaction conditions: 100, 500, 750, or 1000 nM ribosomes, 1 μM mRNA, and 10 μM CBG-549-QSY7. (C) Differential curves for the fluorescence.

This Article

  1. Published in Advance December 18, 2014, doi: 10.1261/rna.047159.114 RNA 21: 296-305

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