Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Properties of nonrandomized siRNAs. (A) Sequences of siRND3 and nonrandomized siRNA3 control duplexes differing in the number of G–C base pairs present (0–7). The sequences of the siRNAs were based on siRND3 and mismatches introduced at the positions where randomizations occurred in siRND3. Watson–Crick base pairs are indicated with a dash. (B) Melting curve analysis of nonrandomized siRNA3 controls. The graph shows the first derivative of melting curves. TMs listed in the table represent the mean of triplicate measurements ± SD. (C) Sequences of the nonrandomized mismatch control siRNA duplexes. The sequences of the siRNAs were based on siRND3 and mismatches introduced at the positions where randomizations occurred in siRND3. Watson–Crick base pairs are indicated with a dash. (D) Melting curve analysis of mismatch controls. The graph shows melting temperatures of nonrandomized siRNA duplexes that carry a defined number of mismatched base pairs. TMs listed in the table represent the mean of triplicate measurements ± SD.

This Article

  1. Published in Advance October 29, 2015, doi: 10.1261/rna.053637.115 RNA 21: 2132-2142

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA