Analysis of the overexpression of the circRNA “Os08circ16564” in transgenic rice plants. (A) Visualization of Os08circ16564 circularization structure and its parental gene (AK064900). A brown bar denotes an exon, a gray bar denotes UTR, and a brown line denotes an intron. Vertical triangles indicate the predicted miRNA osa-miR810b.2 (red) and osa-miR172d-5p (green) binding sites. (B) The qRT-PCR results of Os08circ16564 overexpression lines and empty-vector transgenic lines. Leaf (left panel) and panicle (middle panel) tissues were collected from three independent lines. (E.C.-con) Convergent PCR for empty-vector control transgenic plants; (O.E.-con) convergent PCR for overexpression transgenic plants; (E.C.-div) divergent PCR for empty-vector control transgenic plants; (O.E.-div) divergent PCR for overexpression transgenic plants. (Right panel) The qRT-PCR results of Os08circ16564 by divergent primers in overexpression transgenic plants. Actin was used as a linear control. Leaf and panicle tissues were collected from five independent rice plants. Error bars indicate ± SD. (C) The qRT-PCR results show that the expression levels of Os08circ16564's parental gene AK064900 in both the leaf and panicle tissues of the Os08circ16564-transgenic plants were greatly reduced compared with those of empty-vector transgenic plants. Leaf and panicle tissues were collected from 10 lines. Error bars indicate ± SD. (D) Functional classification of DEGs between transgenic and empty-vector transgenic lines in leaf and panicle.
