Identification and classification of rice circRNAs. (A) Flowchart for identification of circRNAs. (GMAP) A genomic mapping and alignment program. (B) Sensitivity and false-discovery rate estimates. Referring to RAP2 gene models, reads with backsplice sites were simulated. A complete analysis of five individual data sets (∼1500–10,000 designed backsplice sites) yielded a sensitivity of >81.4% and a FDR of <1.7%. (C) Venn diagrams of circRNAs detected in poly(A)-selected and poly(A)-depleted samples of rice leaf and panicle. (D) Genomic origin of rice circRNAs. The numbers in red represent genomic annotation of all 2354 circRNAs referring to RAP2. The numbers in green represent some of all circRNAs that could align in an antisense manner to other genes.
