Activities of the peptidyl transferase center of ribosomes lacking protein L27

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 1.
FIGURE 1.

Characterization of ΔL27 ribosomes. (A) Sucrose-gradient centrifugation profile of wt (upper panel) and ΔL27 (bottom panel) ribosomes at 5 mM Mg2+. (B) Quantification of ribosomal proteins by mass spectrometry. The ratio of the average protein concentrations (as defined by label-free quantification) ΔL27/wt was plotted. Error bars represent the standard deviation of four technical replicates. (C) Western blot of ribosomal proteins from MRE600, wt, and ΔL27 ribosomes using anti-L27 antibody. (D) Determination of the active concentration of ΔL27 ribosomes. The extent of initiation was determined by the radioactivity retained on nitrocellulose filters after incubation of wt (open circles) or ΔL27 (closed circles) ribosomes with mRNA, initiation factors, GTP, and increasing concentrations of f[3H]Met-tRNAfMet.

This Article

  1. Published in Advance October 16, 2015, doi: 10.1261/rna.053330.115 RNA 21: 2047-2052

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA