Depletion of Macpiwi1 but not Macvasa results in stem cell failure. (A) Macpiwi1 and Macvasa immunostaining after luciferase KD (4 wk), Macpiwi1 KD (3 wk), and Macvasa KD (4 wk) in adult worms. EdU-positive cells are shown in red. (t) Testis, (o) ovary, (de) developing egg, (e) egg, (h) head. Stars denote eyes. The arrow denotes nonspecific staining of diatoms in the gut. (B) Macpiwi1 immunostaining in 1-wk-old hatchlings, regenerating worms 3 and 9 d post anterior amputation. EdU-positive cells are shown in red. (h) Head, (rt) regenerating tail. Stars denote eyes. (C) Fold change of differentially expressed apoptosis-related transcripts in Macpiwi1 KD, normalized to luciferase KD. (Yellow dashed line) Fold change = 1. Macpiwi1 is plotted as a control.
