Characteristics of Macpiwi1-associated piRNAs. (A) Arrow points to 5′-end radiolabeling of Macpiwi1-associated small RNAs. (B) Length distribution of sequence abundance and complexity of Macpiwi1-associated small RNAs. Numbers of uncollapsed and collapsed reads per million total mapping reads (RPM) that aligned to the genome are plotted. (C) Distribution of 5′ overlaps between sense and antisense strands of Macpiwi1-associated piRNAs. (D) A heatmap depicting the strand bias of Macpiwi1-associated piRNAs from each transposon consensus sequences. (E) An example of piRNA coverage on a transposon consensus sequence, RXX-Blc11. piRNAs mapping in sense and antisense orientations are labeled in pink and purple, respectively. A close-up view shows ping-pong signature characterized by a 10-bp overlap between sense and antisense strands.
