FIGURE 7.
Level of the mutated 23S rRNA in E. coli cells transformed with pEC23M04 plasmid. (A) Normalized fluorescence intensity of either BiDz0 (open circles) or BiDz-m04 (filled circles) sensor in the presence of mixtures of E. coli cells containing a different amount of the cells transformed with pEC23M04 plasmid. The fluorescence values were normalized by either the averaged fluorescence for all samples (for BiDz-wt) or maximum fluorescence (for BiDz-m04) achieved in the presence of 100% transformed cell cultures. (B) Gel electrophoretic analysis of the products of primer extension assay. (TC) Transformed cells, (NC) nontransformed E. coli cells used as a control.
