Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors

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FIGURE 5.
FIGURE 5.

Quantification of 23S rRNA-04 in the mixtures of wt and m04 23S rRNA transcripts. (A) BiDz assay using either BiDz0 (open circles) or BiDz-m04 (filled circles) sensors. All samples contained 40 pM total 23S rRNA with different fractions of m04 RNA transcript. The values on y-axis represent fluorescence normalized by either the averaged fluorescence for all samples (for BiDz-wt) or maximum fluorescence (for BiDz-m04) achieved in the presence of 100% 23S rRNA-m04. (B) Primer extension assay. The samples contained the same fractions of m04 RNA transcript as in A.

This Article

  1. Published in Advance August 19, 2015, doi: 10.1261/rna.052613.115 RNA 21: 1834-1843

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