Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors

Selectivity of BiDz sensors. Fluorescence intensity of BiDz-wt(15) (dark gray bars) or BiDz-SNS(15) (light gray bars) in the presence of either synthetic oligonucleotide analytes (wt analyte or SNS analyte) (200 pM), 23S rRNA-wt (250 pM), wt E. coli cells or cells spiked with synthetic SNS analyte (200 pM). Blank sample contained no analyte. The results for three independent experiments were averaged; the errors are given as standard deviations.