Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 1.
FIGURE 1.

Principle of the BiDz assay. Two DNA strands, Dza and Dzb, hybridize to the abutting positions of RNA analyte and form a catalytic core that cleaves a fluorophore- and a quencher-labeled reporter substrate. Fluorescence increases due to the spatial separation of the fluorophore from the quencher upon the substrate cleavage.

This Article

  1. Published in Advance August 19, 2015, doi: 10.1261/rna.052613.115 RNA 21: 1834-1843

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA