(A) RT-qPCR analysis of eIF4AII mRNA levels in NIH/3T3[sgROSA] and eIF4AIIem1JP cells following infections with retroviruses expressing shRNAs targeting eIF4AII. (B) siRNA-mediated suppression is unaffected by eIF4AII knockdown in NIH/3T3[sgROSA] or eIF4AIIem1JP cells. Cells were transduced with shRNAs targeting eIF4AII 24 h prior to assessing siRNA-mediated suppression, as described in Figure 3B. (C) Let-7–mediated miRNA repression is unaffected by eIF4AII knockdown. NIH/3T3[sgROSA] or eIF4AIIem1JP cells were transduced with shRNAs targeting eIF4AII 24 h prior to assessing miRNA-mediated suppression, as described in Figure 3D. (D) Representative Western blot of HeLa cells transfected with either nontargeting (siNT) or eIF4AII (si4AII) siRNAs. (E) miRNA-mediated gene repression is not affected by loss of eIF4AII in HeLa cells. HeLa cells were transfected with either eIF4AII siRNAs or a nontargeting siRNA control 24 h prior to assessing siRNA—and miRNA-mediated repression as described above. N = 2 biological replications, with each experiment performed with technical triplicates ± SEM.
