Genome-wide analysis of trans-splicing in the nematode Pristionchus pacificus unravels conserved gene functions for germline and dauer development in divergent operons

Conservation of gene content and function between operons of C. elegans and P. pacificus. (A) Significant overlap between the 1:1 orthologs of C. elegans and P. pacificus that are found within the operons of either species (Fisher's test P-value = 9.90 × 10⁻³⁹). The rectangular boxes represent the set of genes within C. elegans and P. pacificus, and their overlap indicates the 1:1 orthologs (n = 5513). The oval regions represent the corresponding subset of genes that are part of operons in respective species. (B) Operons of Type 1, Type 2, and Type 3 in P. pacificus are highly enriched for germline genes (Fisher's test P-value = 2.43 × 10⁻⁷⁴).