A neutral pH thermal hydrolysis method for quantification of structured RNAs

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FIGURE 1.
FIGURE 1.

The UV absorbance of riboswitch aptamers exhibit the hypochromic effect. Secondary structures and UV melt or cooling curves for (A) the FMN riboswitch aptamer upstream of the impX gene from Fusobacterium nucleatum, (B) the SAM-I riboswitch aptamer upstream of the yitJ gene from Bacillus subtilis (Bs), and (C) the c-di-GMP/GEMM-I riboswitch aptamer upstream of the tfoX gene from Vibrio cholerae. Data shown are independent absorbance measurements of RNA samples from a single transcription reaction.

This Article

  1. Published in Advance May 23, 2014, doi: 10.1261/rna.045856.114 RNA 20: 1153-1160

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