H2-MRB and 3010-MRB copurify with pre-edited and edited mRNAs. (A) Fold-enrichment in IPs of the samples normalized to the mock IP in qPCR assays. (E) Edited, (P) pre-edited (P), (COI) never-edited COI mRNA, two reference transcripts: (TUB) tubulin mRNA and (18S) nuclear 18S rRNA (Fold = 2−ddCq, where ddCq = Cq test IP−Cq mock IP). (B) dCq of steady-state mRNAs in mitochondrial extract relative to background tubulin mRNA or nuclear 18S rRNA used as reference (dCq = 2(Target Cq−Ref Cq)). For example, edited ND7 is more abundant than other edited mRNAs (i.e., has a lower Cq). All end-point amplicons in this study were single products during linear amplification, gel-purified and sequenced. Cq duplicates (STDEV ≤ 0.1) were averaged, and dilutions were adjusted to 100%.
