In-line probing results of the TOM1L2, CBX1, and APC, PG4s possessing central loops of 32, 33, and 30 nt, respectively. (A) Nucleotide sequences of the characterized wt transcripts. The lowercase guanines (g) correspond to those substituted for adenines in the G/A-mutant version. Underlined G-tracks indicate the nucleotides predicted to be involved in the G-quadruplex formation. The boxed sequences denote the predicted PG4. (B–D) K+/Li+ ratios of band intensities of the wt and G/A-mutant for each nucleotide. (B) TOM1L2, (C) CBX1, and (D) APC. The K+/Li+ ratios are shown in dark gray for the wt and in light gray for the G/A-mutant. The boxed guanines represent the predicted G-tracks. The dotted line represents the twofold threshold that denotes a significant gain in flexibility. The nucleotide sequence is indicated on the y-axis. The lowercase Gs shown on the y-axis are mutated to As in the mutant version. Each bar represents the average of two independent experiments, and the error bars represent the standard deviations.
