Exploring conditional alleles and drug treatment with a copper-inducible reporter. A time course (A) of CUP1-Reporter induction is shown. Wild-type cells harboring a CUP1-Reporter were grown to early logarithmic phase and induced with 1 mM CuSO4. Samples were taken at the indicated time points. Yeast with wild-type MEX67 or mex67-5 and the CUP1-Reporter were grown at permissive (31°C) temperature, then shifted to permissive or nonpermissive (34°C or 37°C) temperature with 1 mM CuSO4 (to induce reporter) for 4 h before analysis. Data for cells at permissive temperature are shown in the background in red, with data from cells at nonpermissive temperature in the foreground in blue (B). Pseudo-color phenographs insets are shown for the corresponding nonpermissive conditions. Wild-type cells with CUP1-Reporter and an empty vector or RRP6 overexpression plasmid were grown in minimal media with galactose. Cells were treated with DMSO (vehicle only control) or 100 μM 5-FU, induced with 1 mM CuSO4 and grown for 4 h before analysis (C). The rightmost panel of each set is an overlay of the DMSO and 5-FU data sets for comparison.
