RNAs nonspecifically inhibit RNA polymerase II by preventing binding to the DNA template

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 5.
FIGURE 5.

RNAs inhibit transcript formation when added either before or after basal transcription factors. (A) Varying concentrations of poly(U)50 RNA were added prior to the addition of most of the general transcription factors. Transcription was allowed to proceed for 45 min. (B) A was repeated but with the addition of poly(U)50 RNA after all of the general transcription factors. (C) A and B were performed in triplicate and quantitated.

This Article

  1. Published in Advance March 10, 2014, doi: 10.1261/rna.040444.113 RNA 20: 644-655

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA