FIGURE 4.
Inhibition of Pol II by RNA is not sequence-specific but is size-dependent. (A) Varying lengths of poly(U) RNA were incubated with Pol II for 15 min at 30°C. The Pol II-RNA complex was then incubated with radiolabeled CKO432-CKO433 open DNA for 15 min, and complexes were separated on a 5% native gel. Shifted bands were quantitated in triplicate relative to total signal for each RNA. (B) A was repeated using varying lengths of poly(dT) single-stranded DNA oligonucleotides.
