miRNA sensitivity to Drosha levels correlates with pre-miRNA secondary structure

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FIGURE 9.
FIGURE 9.

In vitro processing of miRNAs using nuclear extract. (A) In vitro-transcribed miRNAs, processed by 293T cell nuclear extract and visualized on a 10% urea-polyacrylamide gel. Bands corresponding to processed pre-miRNAs are marked in gray box, and areas containing pri-miRNAs used for normalization are labeled with black box. (B) 10/90-min ratios of quantified pre-miRNA levels normalized to each respective pri-miRNA levels as indicated in A.

This Article

  1. Published in Advance March 27, 2014, doi: 10.1261/rna.043943.113 RNA 20: 621-631

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