miRNA sensitivity to Drosha levels correlates with pre-miRNA secondary structure

Fold change of overexpression levels of wild-type and mutated versions of miR-145 (A), miR-137 (B), miR-200b (C), and miR-9 (D) between Drosha knockdown and control HeLa cells, assayed by TaqMan probes and normalized to RNU66. (n = 3.) Transfection efficiency was accounted for by SYBRgreen qPCR of the bleomycin resistance gene in the vector, normalized to GAPDH and subtracted from the ▵Ct values from the TaqMan assays. Final normalization was made to the fold change of overexpression for each wt construct. Statistical significance was calculated using a Student's t-test, resulting in P-values of <0.01 (mir-145), <0.05 (mir-137), =0.05 (mir-200b).