FIGURE 4.
Quantitative filtration assay to determine the binding affinities of RF3-nucleotide for pre- and post-release ribosome termination complexes. (A) A representation of the favored ribosome conformations (nonrotated and rotated) in the presence of wild-type and GAQ-RF1. In release complexes containing wild-type RF1, the peptide is released, while in complexes with GAQ-RF1, peptide remains bound and the nonrotated state of the ribosome is favored. (B) Binding of RF3 associated with no nucleotide (▪), GDP (♦), or GDPNP (•) to termination complexes formed with wild-type RF1 or (C) GAQ-RF1.
