FIGURE 1.
Measurements of nucleotide binding to RF3. Individual mant-GDP data points are displayed in gray circles and mant-GTP data are shown as black squares. (A) The change in mant-GDP fluorescence as a function of titrating unlabeled GDP or GTP (0–1000 nM) into solution was monitored on a fluorimeter. The data were fit with equations 1 and 2 to obtain KD values. (B) The change in fluorescence upon mant-GDP/GTP (5 nM) binding to RF3 (0–1000 nM) was observed on a fluorimeter. These data were fit with equation 3 and the fits are displayed.
