Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity

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FIGURE 3.
FIGURE 3.

Concentration optimization of DIG-labeled probe in DSLE. Three different probe concentrations (1 μM, 0.1 μM, and 0.01 μM) were used to perform this assay. Ten micrograms of HeLa RNA was used for each probe concentration: (lane M) mixture of 26 nt and 32 nt 5′-DIG–labeled oligonucleotides, (lane 1) 1 μM probe concentration, (lane 2) 0.1 μM probe concentration, and (lane 3) 0.01 μM probe concentration. (*) Extra bands.

This Article

  1. Published in Advance February 26, 2014, doi: 10.1261/rna.042150.113 RNA 20: 580-584

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