FIGURE 5.
Far-Western dot-blot assay studying the role of each subregion of AnaRneC in AnaPnp–AnaRneC interaction. AnaRneC and its subregion deletion variants were dotted onto duplicate nitrocellulose membranes. Subsequently, one of the membranes was incubated with 2 µM AnaPnp and the other one was not. Both membranes were finally subjected to immunodetection with the antibody against AnaPnp (see Materials and Methods for details). The deleted regions in AnaRneC variants are illustrated with boxes with gray stripes. BSA and AnaPnp were used as the negative control and the positive control, respectively.
