RNase E forms a complex with polynucleotide phosphorylase in cyanobacteria via a cyanobacterial-specific nonapeptide in the noncatalytic region

Comparison of the catalytic properties of EcRneN (the catalytic domain of E. coli RNase E), AnaRne (the full length of Anabaena RNase E), and AnaRneN (the catalytic domain of Anabaena RNase E) on the 9S RNA as substrate. (CK-) 9S RNA incubated for 60 min in the reaction buffer alone. In each of the other reactions, an equal amount of substrate and enzyme were used. The electrophoresis was performed in a 6% PAGE gel containing 7 M urea, and the gel was stained with ethidium bromide. The bands of 9S RNA and its end product p5S RNA are indicated by arrows.