A single inhibitory upstream open reading frame (uORF) is sufficient to regulate Candida albicans GCN4 translation in response to amino acid starvation conditions

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FIGURE 3.
FIGURE 3.

Transcriptional and translational regulation of C. albicans GCN4 expression. (A) The wild-type and gcn2 mutant strains were treated with the indicated concentrations of 3AT for 3 h, and GCN4-Luc activity was measured (RLU). (B) The wild-type and gcn2 mutant were treated with 3AT as above, and GCN4-Luc mRNA levels are expressed relative to GAPDH. (C) GCRE-Luc activity is shown for the wild-type and gcn2 mutant treated with 3AT as above.

This Article

  1. Published in Advance February 25, 2014, doi: 10.1261/rna.042267.113 RNA 20: 559-567

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