A single inhibitory upstream open reading frame (uORF) is sufficient to regulate Candida albicans GCN4 translation in response to amino acid starvation conditions

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FIGURE 2.
FIGURE 2.

Mapping the transcription start site of GCN4 by 5′-RACE. (A) The transcription start site of GCN4 was mapped by 5′-RACE under both nonstressed and following 15 mM 3AT stress for 3 h. (B) The transcription start site is indicated with an arrow on the leader sequence of GCN4. uORF1, uORF2, and uORF3 are indicated with boxes. The positions of the stem–loop structures introduced upstream of and downstream from uORF3 are indicated.

This Article

  1. Published in Advance February 25, 2014, doi: 10.1261/rna.042267.113 RNA 20: 559-567

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