Increased RNAPII transcription rate in rat1-1 cells. (A) Transcription elongation rate assay measuring the last wave of transcription. Cells were grown in AA-Ura/2% raffinose at 30°C to an OD600 of 0.6. The GAL1 promoter was then induced with 2% galactose for 3 h at 30°C. After an additional incubation for 1 h at 34°C, the promoter was turned off by the addition of 2% glucose to the cultures, and samples were cross-linked at the indicated time points. Values correspond to Rpb1p ChIP signals, obtained with the indicated amplicons, at each time point, and normalized to signals in galactose (time point 0). Averages and standard deviations of all ChIP data were calculated from three independent biological experiments, each subjected to triplicate quantitative PCR analysis. (B,C) Growth capabilities measured by 10-fold dilution series of the indicated strains spotted onto YM-1 plates. Strains were grown for 2 d at 30°C or 34°C as indicated.
