Ctk1-HA recruitment at GAL1::YLR454W is increased in rat1-1 cells. Fcp1-myc (A), Ctk1-HA (B), and Rat1-TAP (D) distributions along the GAL1::YLR454W gene as measured by ChIP (solid lines). Parallel Rpb1p ChIP values in the indicated strains are shown as dotted lines. Cells were grown as described in the legend of Figure 2B. ChIP values are relative to the wt signal at the YLR454W promoter region (P). (C) Ctk1-HA ChIP normalized to Rpb1p in wt and rat1-1 strains. Position 8.5 in the wt strain is set to zero since Rpb1 ChIP levels are at background in wt cells (see Fig. 2B). Averages and standard deviations of all ChIP data were calculated from three independent biological experiments, each subjected to triplicate quantitative PCR analysis.
