Association of a peptoid ligand with the apical loop of pri-miR-21 inhibits cleavage by Drosha

Analysis of peptoid 12 binding to RNAs I and II by Mg²⁺-induced cleavage. (A,C) Fluorescent scans of polyacrylamide gel for analysis of 5′ end-labeled RNAs I (A) and II (C) after treatment in the presence of 5 mM MgCl₂ and peptoid. MgCl₂ and peptoid were omitted for the lane labeled “−Mg²⁺.” The peptoid concentration (µM) is shown above lanes containing MgCl₂. OH- and T1 are alkaline hydrolysis ladder and ribonuclease T1 digestion, respectively. (B,D) Cleavage patterns of RNAs I (B) and II (D) in the absence of peptoid and in the presence of peptoid at the indicated concentrations. The lengths of the arrows are semiquantitative indicators of relative cleavage efficiency.