hnRNP A1/A1b and A2, but not B1, can transform PHM-1 cells in vivo. (A,B) PHM-1 cells transduced with retroviruses encoding empty vector (pBABE) or hnRNP A1, A1b, A2, and B1 were analyzed by Western blotting for hnRNP A1/A1b, hnRNP A2/B1, and T7-Tag protein to detect both endogenous and exogenous expression. β-Actin was used as a loading control. (C) Growth curves of cells described in A were measured by methylene blue staining. Error bars, SD (n = 6). (D) Cells described in A were injected (3 × 106 cells/site) subcutaneously near both rear flanks of nude/nude mice, and tumor volume was measured twice weekly (mean ± SEM; n = 16 or 8). (*) P < 0.05, (**) P < 0.001 (two-tailed t-test). (E) Representative mice described in D are shown.
