FIGURE 2.
Effect of shRNA-mediated depletion of selected protein kinases on endogenous Rac1b protein levels. NCM460 cells were transiently transfected with shRNAs against the indicated kinases, lysed after 48 h, and analyzed by Western blot for endogenous Rac1b protein and α-tubulin as loading control. Band intensities from the primary screen blot were determined by densitometry and plotted in the graph below to display fold changes in Rac1b protein expression compared to control cells and identify candidate kinases for subsequent analysis.
