Simple methods for the 3′ biotinylation of RNA

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 1.
FIGURE 1.

RNA tailing with biotin-ATP by S. pombe Cid1 and E. coli PAP. Two micromolar 5′ labeled RNA N22 was incubated with 0.5 mM ATP or N6-biotin-ATP as indicated and the following enzyme concentrations: 1 µM tCid1 (ATP reaction); 5 µM tCid1 (N6-biotin-ATP); 0.25 units/µL E. coli PAP (N6-biotin-ATP); and 0.025 units/µL E. coli PAP (ATP). After the incubation times indicated, aliquots were mixed with 8 M urea in 1× TBE and analyzed by denaturing polyacrylamide gel electrophoresis and phosphorimaging. DNA marker sizes are indicated.

This Article

  1. Published in Advance January 21, 2014, doi: 10.1261/rna.042986.113 RNA 20: 421-427

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA